Culture Collection of Actinomycetes

Research & Outreach

Currently running projects

1. Biology of Hyaluronic acid

OP JAC (2025-2028), project No. CZ.02.01.01/00/23_020/0008499 

Principal Investigator: prof. RNDr. Libor Grubhoffer, CSc., dr. h. c. mult. 

WP1: Structural and functional analysis of microbial systems for hyaluronan production and its enzymatic modification (led by Alica Chroňáková)

team members: 

Msc. Rosa Paulina Calvillo-Medina, Ph.D. (micromycetes)

RNDr. Vladimír Šustr, CSc. (enzymes)

Mgr. Klára Činátlová (technician)

students:

Žofie Podolková (bachelor program Biological Chemistry, Faculty of Science, University of South Bohemia in České Budějovice)

 

Finished projects

1. Microbial degradation of macrolide antibiotics in soil-plant ecosystem treated with wastewater treatment plant products

The Grant Agency of the University of South Bohemia in České Budějovice (2023-2024), project No. 063/2023/P

Principal Investigator: Ing. Lucie Kotrbová

 

2. Unique biosynthetic enzymes as the key to novel bioactive compounds

Ministry of Healthcare of the Czech Republic (2017-2020), No. 17-30091A

Principal investigator: Mgr. Kateřina Petříčková, PhD., University of South Bohemia in České Budějovice, Faculty of Science, České Budějovice

Co-investigators: 

RNDr. Alica Chroňáková, PhD., Biology Centre CAS, Institute of Soil Biology, České Budějovice

Ing. Miroslav Petříček, PhD. The First Faculty of Medicine, Charles University in Prague

 

3. Towards novel bioactive compounds: characterization of specialized metabolites encoded on unique biosynthetic gene clusters

Czech Academy of Sciences - DAAD project (2018-2019), No. DAAD-18-02

Principal investigators:

RNDr. Alica Chroňáková, PhD., Biology Centre CAS, Institute of Soil Biology, České Budějovice

prof. Leonard Kaysser, Ph.D. Eberhard-Karls-Universität Tübingen, Inst. Pharmaceutical Biology, Germany

 

Publications

  • Kotrbová L, Grabicová K, Švecová H, Staňová AV, Petrlíková M, Grabic R, Kodešová R, Chroňáková A. (2025) The effect of WWTP products amendments on Phaseolus vulgaris rhizosphere and its ability to inactivate clarithromycin. Sci Rep. 2025 Aug 22;15(1):30950. http://doi.org/10.1038/s41598-025-14953-6
  • Lara, A. C., Kotrbová, L., Keller, M., Nouioui, I., Neumann-Schaal, M., Mast, Y., & Chroňáková, A. (2024). Lentzea sokolovensis sp. nov., Lentzea kristufekii sp. nov. and Lentzea miocenica sp. nov., rare actinobacteria from Miocene lacustrine sediment of the Sokolov Coal Basin, Czech Republic. International Journal of Systematic and Evolutionary Microbiology, 74(4), 006335. https://doi.org/10.1099/ijsem.0.006335
  • Koubová, A., Lorenc, F., Horváthová, T., Chroňáková, A., & Šustr, V. (2023). Millipede gut-derived microbes as a potential source of cellulolytic enzymes. World Journal of Microbiology and Biotechnology, 39(7), 169. https://doi.org/10.1007/s11274-023-03620-5
  • Bobek, J., Filipová, E., Bergman, N., Čihák, M., Petříček, M., Lara, A. C., Krištůfek, V., Megyes, M., Wurzer, T., Chroňáková, A. & Petříčková, K. (2022). Polyenic Antibiotics and Other Antifungal Compounds Produced by Hemolytic Streptomyces Species. International Journal of Molecular Sciences, 23(23), 15045. https://doi.org/10.3390/ijms232315045
  • Kotrbová, L., Lara, A.C., Corretto, E., Scharfen, J., Ulmann, V., Petříčková, K., & Chroňáková, A. (2022). Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns. Sci Rep 12, 9353. https://doi.org/10.1038/s41598-022-13094-4
  • Lara A. C., Corretto E., Kotrbová L., Lorenc F., Petříčková K., Grabic R., Chroňáková A. (2021). The Genome Analysis of the Human Lung-Associated Streptomyces sp. TR1341 Revealed the Presence of Beneficial Genes for Opportunistic Colonization of Human Tissues. Microorganisms, 9(8), 1547.https://doi.org/10.3390/microorganisms9081547
  • Gorniaková, D., Petříček, M., Kahoun, D., Grabic, R., Zelenka, T., Chroňáková, A., & Petříčková, K. (2021). Activation of a Cryptic Manumycin-Type Biosynthetic Gene Cluster of Saccharothrix espanaensis DSM44229 by Series of Genetic Manipulations. Microorganisms, 9(3), 559.  https://doi.org/10.3390/microorganisms9030559
  • Hrdý, J., Súkeníková, L., Petrásková, P., Novotná, O., Kahoun, D., Petříček, M., Chroňáková, A. & Petříčková, K. (2020). Inhibition of Pro-Inflammatory Cytokines by Metabolites of Streptomycetes—A Potential Alternative to Current Anti-Inflammatory Drugs?. Microorganisms, 8(5), 621.  https://doi.org/10.3390/microorganisms8050621
  • Herbrík, A., Corretto, E., Chroňáková, A., Langhansová, H., Petrásková, P., Hrdý, J., Čihák, M., Krištůfek, V., Bobek, J., Petříček, M. & Petříčková, K. (2020). A human lung-associated Streptomyces sp. TR1341 produces various secondary metabolites responsible for virulence, cytotoxicity and modulation of immune response. Frontiers in Microbiology, 10, 3028. https://doi.org/10.3389/fmicb.2019.03028
  • Petříček, M., Petříčková, K., Pospíšil, S., Kuzma, M., Chroňáková, A., Krištůfek, V., & Stříž, I. (2016). U.S. Patent No. 9,447,062. Washington, DC: U.S. Patent and Trademark Office. U.S. Patent No. 9,447,062. 20 Sep. 2016.
  • Řeháková, K., Chroňáková, A., Krištůfek, V., Kuchtová, B., Čapková, K., Scharfen, J., Čapek, P. & Doležal, J. (2015). Bacterial community of cushion plant Thylacospermum ceaspitosum on elevational gradient in the Himalayan cold desert. Frontiers in Microbiology, 6.   https://doi.org/10.3389/fmicb.2015.0030
  • Petříčková, K., Chroňáková, A., Zelenka, T., Chrudimský, T., Pospíšil, S., Petříček, M., & Krištůfek, V. (2015). Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques. Frontiers in Microbiology, 6.  https://doi.org/10.3389/fmicb.2015.00814
  • Petříčková, K., Pospíšil, S., Kuzma, M., Tylová, T., Jágr, M., Tomek, P., Chroňáková, A., Brabcová, E., Anděra, L., Krištůfek, V. & Petríček, M. (2014). Biosynthesis of Colabomycin E, a New Manumycin-Family Metabolite, Involves an Unusual Chain-Length Factor. ChemBioChem 15, 1334-1345.    https://doi.org/10.1002/cbic.201400068
  • Scharfen, J., Bunček, M., Ježek, P., Urbášková, P., Fridrichová, M., Krištůfek, V., & Chroňáková, A. (2010). Filamentous" contaminants" in the mycobacteriology laboratory; their culture, identification and clinical significance. Klinicka Mikrobiologie a Infekcni Lekarstvi, 16(2), 48-57. https://europepmc.org/article/med/20503156
  • Chroňáková, A., Krištůfek, V., Tichý, M. & Elhottová, D. (2010). Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment. Microbiological Research 165, 594-608.  https://doi.org/10.1016/j.micres.2009.10.002

 

Students' theses

  • BERNAZ, Oana. Evaluating activities suggesting virulence activity in environmental and human-associated actinobacteria. Č. Budějovice, 2025. Bc. Thesis, in English. – 56 p., Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
  • WEISSENGRUBER, Runa. How endophytic actinobacteria respond to pharmaceuticals present in soil. Č. Budějovice, 2024. Bc. Thesis, in English. – 46 p., Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
  • KHALED YOUSSEF SAYED, Sara. Searching for the Polyenes in Streptomyces Associated with Bark Beetles. Č. Budějovice, 2023. MSc Thesis, in English. – 63 P., Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
  • KELLER, Moritz. The secrets encoded in the draft genome of Lentzea sp. BCCO 10_0061 isolated from reclaimed mine heaps. Č. Budějovice, 2023. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
  • PULFERER, Lisa. Occurrence of the cALAs gene in the BCCO Actinomycetes collection and cultivation improvements for the overproduction of secondary metabolites in soil associated Actinomycetes. Č. Budějovice, 2021. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
  • WURZER, Theresa. The interaction of Streptomyces-like bacteria and model microorganisms in secondary metabolite production, motility and hemolytic activities - Experimental. Č. Budějovice, 2019. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
  • JERNEJ, Linda. Genome analysis of Kutzneria sp. strain BCCO 10_1627 and detection of antifungal secondary metabolites. Č. Budějovice, 2019. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
  • KOŠINOVÁ, Anna. Odpověď půdního mikrobního společenstva na aplikaci farmak přítomných v zavlažovacích vodách se zaměřením na irbesartan. Č. Budějovice, 2019. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

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